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Fig. 5 | Molecular Cancer

Fig. 5

From: CircMEMO1 modulates the promoter methylation and expression of TCF21 to regulate hepatocellular carcinoma progression and sorafenib treatment sensitivity

Fig. 5

CircMEMO1 Inhibits HCC Progression by Regulating the EMT Process via the miR-106b-5p/TET1/5hmC Axis. a Huh-7 cells transduced with sh-circMEMO1 or an empty vector were subjected to immunofluorescence. Scale bars, 100 μm. b Western blotting was used to detect the levels of the indicated proteins in Huh-7 and HCCLM3 cells with different circMEMO1 levels. c sh-circMEMO1 expression increased the ability of Huh-7 cells to form tumoursphere structures, while overexpression of circMEMO1 expression decreased the ability of HCCLM3 cells to form tumoursphere structures. d Huh-7 cells infected with a combination of sh-circMEMO1, miR-106b-5p, and TET1-expressing vectors were subjected to a cell invasion assay. Scale bars, 100 μm. e Cell lysates from Huh-7 cells expressing a combination of sh-circMEMO1, miR-106b-5p, and TET1 were subjected to western blot analysis for the indicated proteins. f Genomic DNA purified from Huh-7 cells expressing a combination of sh-circMEMO1, miR-106b-5p, and TET1 was denatured and neutralized. Global 5hmC levels were then measured by a dot blot assay using an anti-5hmC antibody. g Representative HCC cases in tissue microarrays were analysed by an in situ hybridization assay for miR-106b-5p and immunohistochemical staining for TET1, E-cadherin and Vimentin

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