Fig. 4

Rigosertib improves T-cell responses to αPD-1/αCTLA-4 therapy. a Schematic of ICB non-responsive B16F10 melanoma model. b-d Survival of C57BL/6 female mice, day 11 tumor weight post treatment and tumor volume of B16F10 tumors. Mice reached end point and sacrificed when tumors in the experiment exceeded 15 mm in diameter or became perforated. The experiment was terminated when no mice survived in groups other than the RGS + ICB group. e Analysis of synergy. Interaction plot for tumor growth rate with 95% confidence interval by treatment. f Live CD45⁺ leukocytes were concatenated after downsampling to ~ 12,500 events for t-SNE analysis through flow cytometry and T-cell frequency was shown. g Tumor volume of YUMM3.3 tumors in C57BL/6 female mice. Treatment starts at day 10 post tumor cell inoculation. h Spider plots of tumor volume changes overtime for individual YUMM3.3 tumors. i IHC and j-l Flow cytometric analysis of YUMM3.3 tumors at day 17 post-treatment. Data were replicates from one experiment (n = 10 per group)