Fig. 3
From: KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1
KDM6A promotes ARHGDIB transcription in BCa cells. a A Venn diagram comparing depicted 278 genes as potential direct targets of KDM6A in T24 cells based on RNA-seq data. b-d Gene Ontology (GO) analysis of the 278 genes. Dot plots of top 15 most significantly affected categories were shown. Biological Process (b), Molecular Function (c) and Cellular Component (d). e-h KDM6A and ARHGDIB expression levels in indicated cells. mRNA levels were detected by qPCR (e, g, Puro was set as 1, KDM6A vs Puro, shKDM6A vs NC), and protein levels were determined by Western blot (f, h). i Representative IHC images of KDM6A and ARHGDIB in subcutaneous tumours were shown. Scale bars, 50 μm (left) and 20 μm (right). All quantification analyses were based on independent triplicate experiments. Error bars represent SD. **p < 0.01, ***p < 0.001, NS no significant, based on Student’s t test