Fig. 4

ARHGDIB acts as a downstream effector of KDM6A to mediate the inhibition of cell migration and invasion. a and b siRNA mediated ARHGDIB knockdown in T24 cells was verified by Western blot (a) and qPCR (b), siNC was set as 1. c-e Migration and invasion capacities of the indicated cells were determined by wound-healing assays (c), Transwell migration assays (d), and Transwell invasion assays (e). f Kaplan-Meier survival curve for overall survival of the patients with BCa stratified by ARHGDIB expression levels from tissue microarray (Cat No. HBlaU066Su01; ARHGDIB-low, n = 16, ARHGDIB-high, n = 29). g The protein levels of KDM6A and ARHGDIB in indicated cells were assessed by Western blot. h The mRNA levels of KDM6A and ARHGDIB in indicated cells were detected by qPCR, Puro + siNC was set as 1, Puro + siNC vs KDM6A + siNC, Puro + siARHGDIB vs KDM6A + siARHGDIB. i and j Wound healing assays (i) and Transwell invasion assays (j) of the effects of ARHGDIB knockdown on cell migration and invasion. k and l Cell migration assay of bone marrow monocyte-derived macrophages cocultured with conditioned medium (CM) from indicated cells. m The correlation between KDM6A and ARHGDIB mRNA levels in 12 human fresh BCa tissues was assessed by Spearman’s correlation analysis. n The correlation between KDM6A and ARHGDIB protein levels in tissue microarray (Cat No. HBlaU066Su01) was assessed. o Representative IHC images of KDM6A and ARHGDIB in tissue microarray were shown, Scale bar, 500 μm. All quantification analyses were based on independent triplicate experiments. Error bars represent SD. *p < 0.05, **p < 0.01, ***p < 0.001, NS no significant, based on Student’s t test. Kaplan-Meier analysis based on log-rank test