Fig. 5

KDM6A suppresses BCa cell migration and invasion by inhibiting Rac1. a The protein levels of total and active Rac1 (Rac1-GTP) in KDM6A overexpression and knockdown T24 cells were determined by Western blot. b and c The cell motility of indicated T24 cells treated with MBQ-167 (200 nM) or DMSO for 48 h was evaluated by wound healing assays (b) and Transwell invasion assays (c). d Representative image of filopodia staining in indicated cells were shown. Scale bars, 10 μm. e Quantification of the number of filopodia per cell was shown. f The protein levels of total and active Rac1 (Rac1-GTP) in indicated T24 cells were measure by Western blot. g The levels of total and active Rac1 (Rac1-GTP) in indicated cells were determined by Western blot. All quantification analyses were based on independent triplicate experiments. Error bars represent SD. *p < 0.05, **p < 0.01, ***p < 0.001, NS no significant, based on Student’s t test