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Fig. 3 | Molecular Cancer

Fig. 3

From: EYA2 suppresses the progression of hepatocellular carcinoma via SOCS3-mediated blockade of JAK/STAT signaling

Fig. 3

EYA2 suppresses the malignant phenotypes of HCC cells in vitro and in vivo. (A) Western blot analysis of the expression of EYA2 in HCC cells transiently transfected with siRNA or with the overexpression and mutant vectors. The effect of siRNA-mediated knockdown of EYA2 and pcDNA3.1-mediated overexpression of constructs encoding EYA2 wild-type, EYA2(R255K) and EYA2(A510E) on cell proliferation (B), cell invasion (C), clone formation (D) and cell migration (E) of HCC cells in vitro. (F) The effect of EYA2 knockdown, EYA2 wild-type and EYA2(A510E) mutant on HCC tumor growth in nude mice. (G) The scheme of treatment of orthotopic HCC tumors in nude mice by lentivirus-EYA2. (H) Images, volume and weight of the orthotopic liver tumors of the mice. (I) Expression of EYA2 in tumor tissues detected by immunohistochemistry analysis. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001. (B–F, H–I) mean ± SEM, Student’s t-tests

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