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Fig. 3 | Molecular Cancer

Fig. 3

From: The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC

Fig. 3

circACTN4 increases proliferation and suppresses apoptosis of BC cells. a and b The relative expressions of circACTN4 and ACTN4 were detected in BC cells after transfection with overexpressed circACTN4 plasmid or si-circACTN4 by qRT-PCR. c and d Colony formation assay was used to assess cell survival in BC cells transfected with indicated plasmids and siRNA. e and f EdU assay was conducted to detect cell proliferation ability of BC cells (magnification, × 100, Scale bar, 100um). g CCK-8 assay was performed to evaluate cell viability. h Morphological changes of apoptotic cells were observed by Hoechst 33342 staining (magnification,× 400, Scale bar, 50um) after knockdown of circACTN4. i The apoptosis rate was analyzed by flow cytometry after downregulation of circACTN4. j The expressions of apoptosis-related proteins were determined in BC cells transfected with indicated siRNA by Western blot. GAPDH was used as the normalizing gene in the above experiments. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598