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Fig. 4 | Molecular Cancer

Fig. 4

From: The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC

Fig. 4

circACTN4 promotes migration, invasion and cell cycle progression of BC cells. a and b The migration ability of BC cells were detected by wound healing assays (magnification, × 50, Scale bar, 200um) after overexpression or knockdown of circACTN4. c-f Transwell assay was used to measure BC cells invasion and migration abilities after upregulation and downregulation of circACTN4, respectively (magnification, × 100, Scale bar, 100 um) . g and h The expressions of nm23-H1, MMP9 and MMP2 were detected in BC cells transfected with circACTN4 overexpression plasmids and si-circ#2 by western blot. i Cell cycle analysis was executed by flow cytometry after circACTN4 silencing. j The expressions of cell cycle-related proteins were detected in BC cells transfected with indicated siRNA by western blot. GAPDH was used as the normalizing gene in the above experiments. The data are presented as the mean ± SD, *P < 0.05,**P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598