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Fig. 6 | Molecular Cancer

Fig. 6

From: The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC

Fig. 6

circACTN4 blocks interaction FIR with FUBP1. a The relative expression of FIR in 20 pairs of BC tissues and para-cancer tissues was determined by qRT-PCR. b -d Pearson correlation analysis showed that the expression of FIR was negatively correlated with those of circACTN4, MYC and FUBP1. e Co-IP assay showed that FIR could not be pulled down by anti-FUBP1 in MCF-7 cells with transfected with overexpressed circACTN4 plasmid but FIR could be detected in MCF-7 cells with transfected with si-circACTN4. f Chip assay showed that the transfection of BC cells with different concentrations of si-circ#2 affected the binding of FIR and FUSE. g and h The effects of knockdown and overexpression of FIR in BC cells on the expression of MYC were determined by qRT-PCR and western blot. i The expression of MYC was detected after transfection or co-transfection with the indicated vectors and siRNA by qRT-PCR. GAPDH was used as the normalizing gene in the above experiments. The data are presented as the mean ± SD, *P < 0.05,**P < 0.01, ***P < 0.001

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