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Fig. 8 | Molecular Cancer

Fig. 8

From: The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC

Fig. 8

circACTN4 promotes tumorigenesis and metastasis of BC cells. a and b Representative images of xenograft tumors of each group and tumor weight analysis were shown. c The tumor volumes were measured once a week and the growth curves were drawn. d and e HE staining of lung and tumor sections were showed. Metastatic nodules of the lungs and microvessels of the tumors were analyzed and indicated with arrows (magnification, × 100, Scale bar, 100 μm). f Western blot analysis was used to detect the protein level of MYC in xenograft tumor tissues. g Representative bioluminescent imaging (BLI) of the different groups was shown. circACTN4 knockdown and overexpression MCF-7 cells were injected into tail vein of mice for 4 weeks to detect metastasis (n = 5 for each group). h The survival curve was generated for the nude mice injected with MCF-7 cells infected with overexpressed circACTN4 or mock vector using Kaplan–Meier survival analysis (n = 10 for each group). i The representative images of liver metastasis were taken from nude mice injected with circACTN4 overexpressing and mock control MCF-7 cells for 60 days (magnification, × 400, Scale bar, 100 μm). j The expressions of MYC, CDK4, CCND2 and Ki67 in tumors of nude mice were detected by immunohistochemistry (magnification, × 200, Scale bar, 200 μm). k The schematic diagram shows how circACTN4 could promote tumorigenesis and metastasis of BC through circACTN4/FUBP1/MYC axis. GAPDH was used as the normalizing gene in the above experiments. The data are presented as the mean ± SD, *P < 0.05,**P < 0.01, ***P < 0.001

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