Fig. 4

DIDO1-529aa interacts with PARP1. a) Co-IP analyses of the interaction between DIDO1-529aa and PARP1. b) Co-localization of DIDO1-529aa and PARP1 in the nucleus was examined by immunofluorescence. Scale bars = 25 μm. c) DIDO1-529aa-Flag vector was co-transfected with distinct HA-tagged PARP1 deletions (1–372, 373–524, and 525–1014 aa) into 293 T cells. The interaction of DIDO1-529aa with different PARP1 mutants was tested by Co-IP. DIDO1-529aa was marked in multiple colors and PRPA1 was marked in yellow. The bottom figure showed the structure of DIDO1-529aa-PARP1 interaction. The possible interacting amino acids in DIDO1-529aa were marked in cyan and those in PRPA1 marked in brown. d) Immunofluorescent staining of γ-H2AX in control or DIDO1-529aa overexpressing GC cells. Scale bars = 50 μm. e) Western blot analyses for the expression of cleaved PARP1 and caspase-3 in control and DIDO1-529aa overexpression GC cells