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Fig. 6 | Molecular Cancer

Fig. 6

From: CircDIDO1 inhibits gastric cancer progression by encoding a novel DIDO1-529aa protein and regulating PRDX2 protein stability

Fig. 6

CircDIDO1 interacts with PRDX2 protein in GC cells. a) circDIDO1-binding proteins were identified by TRAP experiment and LC–MS/MS analysis. GST protein expression was detected by Western blot and circDIDO1 expression was detected by qRT-PCR. The binding of circDIDO1 to PRDX2 protein was validated by TRAP experiment followed by Western blot. b) The potential binding site of PRDX2 in circDIDO1 was predicted by catRAPID and verified by Western blot. c) Co-localization of circDIDO1 and PRDX2 protein in GC cells was determined by RNA-FISH and immunofluorescence. Scale bars = 25 μm. d) Western blot and PCR analyses of PRDX2 expression in control or circDIDO1 overexpressing GC cells. e) Heatmap of differentially expressed genes in control and circDIDO1 overexpressing HGC-27 cells. f) Enrichment of differentially expressed genes in different pathways by Pathway analysis. g) The expression of proteins associated with PRDX2 downstream signaling pathways in control and circDIDO1 overexpressing GC cells were detected by Western blot. h) TOP/FOP flash luciferase assay for control and circDIDO1 overexpressing GC cells. Data are shown as means ± SD (n = 3, **P < 0.01)

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