Fig. 4

Evolution of SNVs and Large-scale CNAs in Primary Tumour cells and CTCs. A Schematic diagram of the manner in which primary tumour cells intravasate and become CTCs. B SNVs of primary tumour cells and CTCs. The distribution of 20 non-synonymous mutations was assessed in 28 primary tumour cells (Cells 1–28) and five CTCs (CTCs 1–5) from a colon cancer patient (blue box, mutant; grey box, wild type). Three clones of cells were present according to a probabilistic modelling-based approach. C CNA patterns of the primary tumour, one control leukocyte (C1), single primary tumour cells, CTCs, and three lymph node metastases (Pri., primary tumour; Meta., metastases). The copy numbers (blue and red dots) are plotted along the genome at a bin size of 500 kb. The ordinate coordinate represents copy numbers ranging from 0 to 6 (a copy number of more than 6 copies is set to 6). Phylogenetic tree on the left was constructed based on the segmented copy numbers of single cells [70]. The copyright of this image belongs to Reference [70]