From: Using single-cell sequencing technology to detect circulating tumor cells in solid tumors
Sorting method | Sorting objects | time consuming | Advantage | shortcoming | Reference |
---|---|---|---|---|---|
Microoperation separation method | Less tissue and cell suspension | long | High accuracy and low cost | Time consuming, low flux, easy to cause mechanical damage to the target cells | |
Microfluidic separation | Cell suspension with numerous cells | short | High flux, small reaction volume, less space and less pollution | High cost and high cell loss rate | |
DEPArray sorting system | CTO solution | long | Visualization and semi automation | The sample size is small and can not be directly derived from it | |
 |  |  |  | Separation and enrichment of CTC in peripheral blood |  |
CellCelector sorting system | CTC suspension | short | Keeping cell activity, high accuracy and less time consuming | High cost and high instrument dependence | [42] |