Using single-cell sequencing technology to detect circulating tumor cells in solid tumors

Xu, Jiasheng; Liao, Kaili; Yang, Xi; Wu, Chengfeng; Wu, Wei

doi:10.1186/s12943-021-01392-w

Molecular Cancer

Table 2 Summary of single-cell sequencing studies of various cancer CTCs

From: Using single-cell sequencing technology to detect circulating tumor cells in solid tumors

Tumourtype	Cases	Blood sampling method	CTC enrichment method	CTC			Sequencing method	Analysis Sample			Target gene / sequence	Mutation type	Ref
				CTC single chipCell sorting method	Single			Primary focus	CTC	Metastases / drenchingFawn on
				CTC single chipCell sorting method	Cell Analysis number	WGA method		Primary focus	CTC	Metastases / drenchingFawn on
Mammary cancer	15+43	CellSave	CellSearch	DEPArray	37+202	Amplil™ WGAKit	qPCR/aCGH/Sanger	√	√	X	Erbb2/pik3ca/ allGenomic aCGH analysis	Copy number variationAnomaly / point mutation	[41]
Mammary cancer	2	CellSave	CellSearch	DEPArray	11	Amplil™ WGAKit	Sanger	√	√	X	TP53	point mutation	[7]
Mammary cancer	18	CellSave	CellSearch	DEPArray	115	Amplil™ WGAKit	Sanger	√	√	X	PIK3CA	point mutation	[60]
colorectal cancer	6	CellSave	CellSearch	Micromanipulation	37	GenomePlexSingle Cell WGA kit/ GenomiPhiDNAAmplification kit	aCGH/NGS	√	√	(metastases)	Whole genome aCGHAnalysis of /68 colorectal cancerGuan gene	Copy number variationAnomaly / point mutation	[35]
colorectal cancer	5	CellSave	CellSearch	Micromanipulation	69	GenomePlexSingle Cell WGAkit	Sanger	X	√	X	BRAF/KRAS/PIK3CA	point mutation	[6]
Mammary cancerLung cancer	3+2	EDTA	CTC-Chip + doubleCoating of nanomaterialsGel matrix	frequency controlMicroabsorption	NA	NO WGA	Sanger	√	√	X	PIK3CA/EGFR	point mutation	[64]
MalignancyPigmented tumor	11	Heparinized blood	Dynabeads	Laser captureMicrodissection	14	NO WGA	Nested PCR+ Sanger	X	√	X	BRAF/KIT	point mutation	[61]
Mammary cancer	17	EDTA	MagSweeper	Under microscopeMicro absorption	185	NO WGA	Nested PCR+ Sanger	√	√	(metastases)	PIK3CA	point mutation	[65]
Mammary cancer	5	CellSave	CellSearch	DEPArray	≥100	Amplil™ WGAKit	NGS	√	√	X	ESRI, PIK3CA>TP53 and erbB2 Point mutation; Custom Cancer Hotspot Panel v2 for Amplil WGA DNAfever	point mutation	[66]
pancreatic cancer	12	ACDSolution Atubes	Dynabeads	Nano Velcro/LCM CTCChip	222	REPLI-g SingleCell Kit	Sanger	X	√	X	KRAS	point mutation	[67]
Colon cancer	21	EDTA	Oncoquick	DEPArray	NA	Amplil™ WGAKit	Sanger/pyrosequencing	√	√	X	KRAS	point mutation	[43]
Prostatecancer	1	EDTA	PN-Nano Velcrochip	Laser captureMicrodissection	25	GenomePlexSingle Cell WGAkit	NGS	X	√	X	Whole genome exon measurementorder	point mutation	[68]
Prostatecancer	1	Anticoagulated bloodtubes	Red cell lysis + selfSlide adhesion	Micromanipulation	41	GenomePlexSingle Cell WGA kit	NGS	X	√	X	whole genome sequencing	Copy number variationdifferent	[63]
ProstateCancer /	2	EDTA	MagSweeper/CelISearch	Micromanipulation	25	MDA	NGS	√	√	(lymph node)	Whole genome exon measurementorder	point mutation	[8]
lung cancer	4	CellSave	Cell Search	Under microscopeMicro absorption	24	MALBAC	NGS	√	√	(metastases)	Whole genome exon measurementorder	Copy number variationHetero / point mutationinsert	[9]
Colon cancer	2	CellSave	CellSearch	Under microscopeMicro absorption	5	MALBAC	NGS	√	√	(lymph node)	whole genome sequencing	Copy number variationHetero / point mutationStructural variation	[62]

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ISSN: 1476-4598

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