Fig. 1

Profiling of circRNAs in bone marrow from primary pediatric AML patients and normal controls. A A scatter plot showing the variation in pediatric AML patients and controls. The red plot indicates circRNAs with greater than 2.0-fold upregulation between the two compared samples, while the green plot indicates the downregulated circRNAs. The star symbol denotes circRNF220. B-C The distribution of differentially expressed circRNAs on chromosomes. D Genomic origins of human circRNAs from primary pediatric AML patients. E The length distribution of circRNAs in pediatric AML. F A heat map showing the top 10 upregulated and downregulated circRNAs in the case group vs. the control group. Each row corresponds to a circRNA. High expression levels are indicated by red, and lower levels are indicated by green. G The expression levels of circRNF220 in AML patients were significantly higher than those in the corresponding controls. H qRT–PCR analysis of circRNF220 expression in various mouse organs and tissues. I Violin plot showing the relative abundances of circRNF220 in several hematologic malignancies. Data are expressed as log2 fold change values. The centerline in the box plot indicates the median, and the black dot indicates an abnormal value. AML, acute myeloid leukemia; ALL, acute lymphocytic leukemia; ITP, idiopathic thrombocytopenic purpura; Others, diseases including anemia, thrombocytopenia, chronic myeloid leukemia, lymphoma, juvenile myelomonocytic leukemia, and myelodysplastic syndrome. **P < 0.01, *** P < 0.001