Fig. 2

Identification and characterization of circRNF220 in pediatric AML. A Structures of the RNF220 genomic locus and transcript. CircRNF220 is produced by exon 2. The junction point of circRNF220 was identified by Sanger sequencing. B Divergent primers were used to amplify circRNF220 from cDNA but not genomic DNA (gDNA). Convergent primers amplified both circRNF220 and the linear GAPDH RNA in cDNA and gDNA. C qRT–PCR analysis of the abundances of circRNF220 and RNF220 mRNA in AML cells treated with actinomycin D at the indicated time points. D qRT–PCR analysis of the abundances of circRNF220 and RNF220 mRNA in AML cells treated with RNase R, with normalization to the value measured in the mock treatment sample. E RNA fluorescence in situ hybridization analysis of circRNF220 in 293 T and AML cells. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). F qRT–PCR data indicating the abundances of circRNF220 and RNF220 mRNA in the cytoplasm and nucleus of 293 T and AML cells. The abundances of circRNF220 and RNF220 mRNA were normalized to the values measured in the cytoplasm. The data in (C-D) and (F) are the mean ± SD, which carried out in cell lines for three times, or in more than 3 primary pediatric AML cases BM cells. *P < 0.05, **P < 0.01