Fig. 4From: CircRNF220, not its linear cognate gene RNF220, regulates cell growth and is associated with relapse in pediatric acute myeloid leukemiaThe requirement for circRNF220 in AML cells. A Effect of circRNF220 overexpression on the proliferation of HL-60 and THP-1 cells. B-C Flow cytometric analysis of cell cycle progression in AML cells. The numbers indicate the percentages of cells. D Microscopic analysis of Wright-Giemsa-stained cytospin preparations of THP-1 cells. Original magnification, 1000 × . E Flow cytometric analysis of myeloid differentiation of THP-1 cells. F The numbers indicate the percentages of differentiated cells. G Flow cytometric analysis of apoptosis in HL-60 and THP-1 cells. H Apoptosis was detected by TUNEL in K562 cells. I The numbers indicate the percentages of apoptotic cells in AML cell lines. J Flow cytometric analysis of apoptosis in primary AML patient cells. K The numbers indicate the percentages of apoptotic cells in primary AML BM cells. * P < 0.05, **P < 0.01Back to article page