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Fig. 3 | Molecular Cancer

Fig. 3

From: N6-methyladenosine-modified circIGF2BP3 inhibits CD8+ T-cell responses to facilitate tumor immune evasion by promoting the deubiquitination of PD-L1 in non-small cell lung cancer

Fig. 3

METTL3-mediated m6A modification promotes back-splicing of circIGF2BP3. A Correlation between circIGF2BP3 expression and linear mRNA expression in 68 paired samples of NSCLC in cohort I from Tongji Hospital. B The relative expression of circIGF2BP3 (left) and linear IGF2BP3 (right) in 68 paired samples of NSCLC in cohort I from Tongji Hospital. GAPDH was used as the internal control. C The relative expression of circIGF2BP3 and linear IGF2BP3 in 4 NSCLC cell lines. GAPDH was used as the internal control. D Venn diagram showing candidate m6A sites in circIGF2BP3 that were predicted by both SRAMP and RMBase. E MeRIP-qPCR analysis of the abundance of m6A-modified circIGF2BP3 in four NSCLC cell lines. F The relative expression of circIGF2BP3 in A549 and SW900 cells after treatment with MAO-circIGF2BP3 or MAO-NC, as detected by qRT-PCR. G The relative expression of circIGF2BP3 in A549 cells after treatment with siMETTL3 or siControl, as detected by qRT-PCR. H MeRIP-qPCR analysis of the relative abundance of m6A-modified circIGF2BP3 in NSCLC cells treated with siMETTL3 or siControl. I METTL3 IP assay to detect circIGF2BP3 in A549 cells. J The relative expression of circIGF2BP3 in siMETTL3 or siControl A549 cells after treatment with MAO-circIGF2BP3 or MAO-NC, as detected by qRT-PCR. K-L Correlation between METTL3 expression and tumor purity (left) and the infiltration level of CD8+ T cells (right) in LUSC (K) and LUAD (L) from a TCGA data set analyzed by the TIMER algorithm. M Correlation between circIGF2BP3 and METTL3 expression in 68 paired samples of NSCLC in cohort I from Tongji Hospital. GAPDH was used as the internal control. N The relative expression of circIGF2BP3, linear IGF2BP3, and IGF2BP3 pre-RNA in A549 cells treated with siControl or siYTHDC1, as measured by qRT-PCR. O YTHDC1 IP assay to detect circIGF2BP3 in A549 cells. P The relative expression of circIGF2BP3 in siYTHDC1 or siControl A549 cells after treatment with MAO-circIGF2BP3 or MAO-NC, as detected by qRT-PCR. Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P values were determined by unpaired Student’s t test (E, F, G, H, J, N and P). Correlations were determined by the Pearson (A and M) or Spearman correlation test (K and L)

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