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Fig. 1 | Molecular Cancer

Fig. 1

From: Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

Fig. 1

Discovery of oncogenic circRNAs and characterization of circEYA3 in PDAC A. Volcano plots showing the upregulated (red) and downregulated (blue) circRNAs in PDAC from GSE79634 and GSE69362 (fold change > 2 or < 0.5, P < 0.01). B Venn diagram exhibiting the overlap of the upregulated circRNAs in GSE79634 and GSE69362. C Schematic illustration presenting the formation of circEYA3 from exons 2–6 of the EYA3 gene. Sanger sequencing verified the existence of circEYA3. The red arrow indicates the back-splicing junction sites in exons 2 and 6 of the EYA3 gene. D The relative expression level of circEYA3 in PDAC cells and HPDE cells was determined by qRT-PCR. E qRT-PCR was used to evaluate the expression of circEYA3 in paired PDAC and matched adjacent normal tissues (n = 104). F Divergent (red) and convergent (blue) primers were used to amplify the back-spliced and linear products to verify the existence of circEYA3 using PANC-1 and MiaPaCa-2 cells. G qRT-PCR and subsequent nucleic acid electrophoresis were used to analyse the expression of circEYA3 and EYA3 mRNA after treatment with RNase R. H Alterations in the circEYA3 and EYA3 mRNA levels were analysed by qRT-PCR under treatment with the transcription inhibitor ActD. I FISH was conducted to detect the distribution of circEYA3 in PDAC cells. Red fluorescence (Cy3-labelled probe) indicates circEYA3. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. All data are shown as the mean ± SD values. *P < 0.05, **P < 0.01, ***P < 0.001

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