Fig. 7

Knockdown of circEYA3 suppresses tumor growth in vivo. A. Stably transfected PANC-1 cells from different groups were inoculated into BALB/c nude mice to establish subcutaneous xenograft tumors (n = 5 mice/group). Representative images of tumor-bearing mice. In addition, tumor volumes were monitored weekly. B. The excised tumors were photographed and weighed. C. The relative expression levels of circEYA3, miR-1294 and c-Myc in subcutaneous tumor tissues were determined by qRT-PCR. D. The c-Myc, E-cadherin, N-cadherin, Vimentin, Snail, Bax, cleaved caspase-3 protein levels in tumours from different groups were determined by western blot analysis. E. IHC staining showed the relative levels of c-Myc, E-cadherin, N-cadherin, Bax, cleaved caspase-3, and Ki-67 in tumours from different groups. Scale bar, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001; ns indicates no significance