Fig. 2

Consequences of ADAR1 knockdown on alternative splicing. A Venn diagram representing the significant (FDR < 0.05 and Inclusion Level Difference < -0.2 / < 0.2) alternative splicing events differing between siControl and siADAR1 #1 (blue) or siADAR1 #2 (orange) in Cal62 cells. One hundred and fourteen events were common in both analyses. B Left panel: Schematic of the alternative splicing events analyzed. Right panel: Pie chart of events with significantly different inclusion levels (FDR < 0.05 and Inclusion Level Difference < -0.2 / < 0.2) upon ADAR1 knockdown. Abbreviations: SE, skipped exon; RI, retained intron; MXE, mutually exclusive; A5SS, alternative 5’ splice site; A3SS, alternative 3’ splice site. C Functional annotation for the genes that present with significant differential alternative splicing events after ADAR1 silencing. D RT-qPCR validation of ADAR1-regulated alternative splicing events for indicated genes 72 h after siRNA transfection in Cal62 cells (n = 4). Error bars indicate standard deviations. Asterisks denote statistical significance compared with siControl treatment as assessed with Student´s t-test (two-tailed). * p < 0.05, ** p < 0.01, *** p < 0.001