Fig. 4

CDK13 is functionally important for thyroid cancer development. Cal62 and TPC1 cells were transfected with three independent siRNAs against CDK13 or a control siRNA (siControl). A CDK13 RNA relative levels (n = 3). B Representative western blotting for CDK13. GAPDH was used as loading control. C Proliferation by BrdU incorporation (n = 3). D and E Viability assays tested by XTT dye reduction (n = 3) (D) or crystal violet staining (n = 4) (E). F Matrigel Transwell invasion assay (n = 3). Error bars indicate standard deviations. Asterisks denote statistical significance compared with siControl treatment as assessed with Student’s t-test (two-tailed). * p < 0.05, ** p < 0.01, *** p < 0.001