Fig. 2

circPLCE1 inhibits CRC cell proliferation and metastasis in vitro. A Colony formation assays of circPLCE1 transfected HCT8 and DLD1 cells, n = 3. B Sphere formation assays of circPLCE1 transfected HCT8 and DLD1 cells, n = 3. Scale bar = 500 μm. C Anchorage-independent growth of circPLCE1 transfected HCT8 and DLD1 cells, n = 3. Scale bar = 200 μm. D Patient-derived organoids (PDOs) growth with circPLCE1 transfection, n = 3. Scale bar = 200 μm. E migration assays of circPLCE1 transfected HCT8 and DLD1 cells, n = 3. Scale bar = 100 μm. F wound-healing assays of circPLCE1 transfected HCT8 and DLD1 cells, n = 3. Scale bar = 100 μm. G Colony formation assays of circPLCE1 knockdown HCT8 and DLD1 cells, n = 3. H Sphere formation assays of circPLCE1 knockdown HCT8 and DLD1 cells, n = 3. Scale bar = 500 μm. I Anchorage-independent growth of circPLCE1 knockdown HCT8 and DLD1 cells, n = 3. Scale bar = 200 μm. J migration assays of circPLCE1 knockdown HCT8 and DLD1 cells, n = 3. Scale bar = 100 μm. K wound-healing assays of circPLCE1 knockdown HCT8 and DLD1 cells, n = 3. Scale bar = 100 μm. Values are represented as mean ± SD. **p < 0.01, ***p < 0.001, by 2-tailed Student’s t test (A-F) and one-way ANOVA (G-K)