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Fig. 5 | Molecular Cancer

Fig. 5

From: Circular RNA circRNF13 inhibits proliferation and metastasis of nasopharyngeal carcinoma via SUMO2

Fig. 5

CircRNF13 inhibits glycolysis in NPC cells by promoting GLUT1 ubiquitination. A The mRNA levels of GLUT1, HK2, and LDHA in HNE2 and CNE2 cells were examined using RT-PCR, upon overexpression and knockdown of circRNF13. Data have been represented as mean ± SD; ns, no significance; ***, p < 0.001. B The expression levels of GLUT1, HK2, and LDHA proteins were examined using western blotting in HNE2 and CNE2 cells, upon overexpression and knockdown of circRNF13. C Degradation of GLUT1 was detected in HNE2 and CNE2 cells using western blotting, after overexpression and knockdown of circRNF13 and treatment with 50 μg/mL cycloheximide (CHX). D Ubiquitination levels of GLUT1 protein were detected in HNE2 and CNE2 cells using pull-down with an anti-Flag antibody and western blot with an anti-ubiquitin antibody, after overexpression or knockdown of circRNF13

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