Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: Circular RNA circRNF13 inhibits proliferation and metastasis of nasopharyngeal carcinoma via SUMO2

Fig. 6

CircRNF13 directly binds to and stabilizes SUMO2 mRNA, to upregulate its expression A. Expression of SUMO2 protein was examined using western blotting in HNE2 and CNE2 cells, after overexpression and knockdown of circRNF13. B Expression of SUMO2 mRNA was examined in HNE2 and CNE2 cells using RT-PCR, after overexpression and knockdown of circRNF13. C Bioinformatics prediction using the RNAhybrid website revealed that circRNF13 has a continuous binding site with the 3′-UTR of SUMO2 mRNA. circRNF13 was found to bind to the 3′-UTR of SUMO2 mRNA in HNE2 and CNE2 cells using RNA pull-down assay. GADPH was used as a negative control. Data have been represented as mean ± SD. ***, p < 0.001. EcircRNF13 enhanced the luciferase activity of the SUMO2 mRNA 3′-UTR in HNE2 and CNE2 cells, as assessed using dual luciferase reporter assay. The 3′-UTR of GADPH was used as a negative control. Data have been represented as mean ± SD. *, p < 0.05; **, p < 0.01. F. Degradation of SUMO2 was detected in HNE2 and CNE2 cells using RT-PCR, after overexpression and knockdown of circRNF13 and treatment with 2 μg/mL actinomycin D. Data have been represented as mean ± SD. ***, p < 0.001, ****, p < 0.0001

Back to article page