Fig. 1

CircMYH9 was upregulated in CRC tissues and predicted poor prognosis. A-B Heat maps of differentially expressed circRNAs in CRC tissues and adjacent normal tissues obtained from GSE126095 (A) and 3 CRC patients (B). C Venn diagram showing the numbers of overlapping circRNAs between the differentially expressed circRNAs in GSE126095 and 3 CRC patients. D Schematic diagram of the genomic location of circMYH9 derived from its parent gene MYH9. E The panel shows the detection of circMYH9 using divergent and convergent primers from cDNA or gDNA of HCT116 cells. F CircMYH9 was further validated by Sanger sequencing. G The relative expression levels of circMYH9 were assessed by qRT-PCR in 53 paired normal tissues and CRC tissues (*, P < 0.05; **, P < 0.01). H Immunofluorescence analysis of circMYH9 expression in peritumour tissues and CRC tissues. I-J Kaplan–Meier analysis of the RFS and OS rates in 148 CRC patients with high or low expression of circMYH9