Fig. 1

Higher frequency of lung tumorigenesis in aged mg53^-/- mice. A IHC and pathological analysis revealing the presence of abnormal cell proliferation in the lung tissue derived from aged mg53^-/- mice (10-months or older) (left panel, Masson Trichrome stain); DAPI indicates nucleus and anti- Ki67 serves as a proliferation marker for human tumor (right). A total of 8 (8/20) lung tissues from mg53^-/- were observed with abnormal cell proliferation. B Tumor growth assay was examined in mg53^-/-, tPA-MG53, and WT mice as described in materials and methods. After 4 weeks, the primary tumors were excised, weighted, and photographed. C Quantification of tumor weight from mg53^-/-, tPA-MG53, and WT mice. Each dot represents an individual mouse, with a line demarcating the median for each cohort with S.D. ** p<0.01