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Fig. 3 | Molecular Cancer

Fig. 3

From: MG53 suppresses tumor progression and stress granule formation by modulating G3BP2 activity in non-small cell lung cancer

Fig. 3

TRIM domain of MG53 interacts with G3BP2 in NSCLC cells. A Schematic representation of the domain structures and the encoding amino acid numberings of MG53 (FL) and its N-terminal or C-terminal construct. B HEK293 cells were transfected with FL-MG53, N-MG53, and C-MG53 for 24 hr. The whole cell lysate was subjected to western blot assay using anti-HA and anti-G3BP2 (left). The immunoprecipitation (IP) assay using anti-HA-conjugated magnetic beads, then performed with anti-HA (right, top panel) and anti-G3BP2 (right, bottom panel). C A549 cells were transfected with RFP-MG53 and G3BP2-GFP for 24 hr. The nucleus (blue) was labeled with Hoechst 33342. The co-localization of MG53 and G3BP2 was examined under confocal microscopy (top). ATO treatment induces nuclear translocation of both RFP-MG53 and G2BP2-GFP (bottom)

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Molecular Cancer

ISSN: 1476-4598