Fig. 4

Knockdown MG53 enhances cell proliferation and migration in NSCLC cells. shRNA lentivirus was used to create stable sh-scramble control (Scr), shMG53, and shG3BP2 knockdown H2122 cells. The whole cell lysates were loaded for western blot and probed for (A) MG53 and (B) G3BP2 expression. Tubulin served as a loading control. C Expression G3BP1 and G3BP2 in Scr control, shMG53, shG3BP1, and shG3BP2 cell lines. GAPDH serves as loading control. H2122 cells with stable knock-down of MG53 or G3BP2 or the control cells expressing scramble constructs were performed with colony formation assay and (D) trans-well assay (F). Quantification of clone formation (E) and trans-well assay (G). ** P<0.01 and *** P<0.005 for the indicated group. H Representative images of Scr control and shG3BP2 cells with 72-hour incubation of BSA (control) or rhMG53 (20 μg/ml). I MTT assays of Scr control and shG3BP2 cells reveal different dose-dependent effect of rhMG53 on cell proliferation. * indicate significant difference with p<0.001. Data represents 3 independent experiments