Fig. 1

AMPK deficiency in Tregs promotes tumor growth. B16F10 melanoma cells were injected subcutaneously into C57BL/6 J mice and analyzed after 22 days. A Analysis of total LKB1 and AMPK in Tregs obtained from tumor-free and tumor-bearing C57BL/6 J mice by western blotting. B Detection of the mRNA expression of the prkaa1 gene by real-time PCR in Tregs isolated from tumor-free and tumor-bearing C57BL/6 J mice. The C tumor volume and D Tumor weight in WT and AMPK^fl/flFoxp3-Cre mice injected s.c. with B16F10 melanoma cells. E Flow cytometric analysis of the percentage of CD4⁺ and CD8⁺ T cells; CD4⁺Foxp3⁺ Tregs and Tregs cellularity in the tumors of WT and AMPK^fl/flFoxp3-Cre mice. F Analysis of IFN-γ-producing CD4⁺ and CD8⁺ T cells percentage in tumors from WT and AMPK^fl/flFoxp3-Cre mice by flow cytometry. G Flow cytometry analysis of GZB-producing CD8⁺ T cells in tumors from WT and AMPK^fl/flFoxp3-Cre mice. H Evaluation of in vivo cytolytic activity in the spleen and draining lymph node of WT and AMPK^fl/flFoxp3-Cre mice. The data are presented as the mean ± standard deviation (SD); n = 5 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001