Fig. 5

p113 facilitates tumorigenesis and aggressiveness of NB cells via interacting with ZRF1. a and b Representative images (left panel) and quantification (right panel) of soft agar (a) and matrigel invasion (b) assays indicating the growth and invasion of NB cells stably transfected with empty vector (mock) or ecircCUX1, and those co-transfected with sgRNA specific against ZRF1 for CRISPRi. c Representative images (upper panel), in vivo growth curve, and weight at the end points (lower panel) of xenografts in nude mice formed by subcutaneous injection of SH-SY5Y cells stably transfected with mock or ecircCUX1, and those co-transfected with sgRNA specific against ZRF1 for CRISPRi (n = 5 for each group). d Representative images (upper panel) and quantification (lower panel) of immunohistochemical staining showing expression of Ki-67 and CD31 within xenografts (n = 5 for each group). Scale bars: 50 μm. e and f Representative in vivo images (e), hematoxylin & eosin staining (f, upper panel), lung metastatic counts (f, lower panel), and Kaplan–Meier curves (f, lower panel) of nude mice treated with tail vein injection of SH-SY5Y cells stably transfected with mock or ecircCUX1, and those co-transfected with sgRNA specific against ZRF1 for CRISPRi (n = 5 for each group). Scale bars: 100 μm. ANOVA compared the difference in a-d and f. Log-rank test for survival comparison in f. *P < 0.05 vs. mock+CRISPRi-Scb. Data are shown as mean ± s.e.m. (error bars) and representative of three independent experiments in a and b