Fig. 2
Tspan8 protein expression can be immunoscored in primary melanomas and correlates with a higher propensity to give rise to distant metastases, an increased risk of death and the presence of a BRAFV600E mutation. a Tspan8 expression detected by immunochemistry in different melanoma samples from immunoscore 1 to 4 (4 first pictures). b, Repartition of primary melanomas according to the Breslow Index (inferior to 1 mm, between 1 and 3 mm, superior to 3 mm), the patient status 5 years after diagnosis (dead or alive) and Tspan8 protein expression (positive or negative): for each category, the percentage of Tspan8+ tumors are presented (n = 100). Fisher’s exact test. c Tspan8 immunostaining classified in 5 different immunoscores from no Tspan8 staining (score 0) to strong Tspan8 staining (score 4), according to the Breslow Index (inferior to 1 mm, between 1 and 3 mm, superior to 3 mm) and the patient status 5 years after diagnosis (dead or alive; n = 100). d Tumor number according to metastasis presence versus TSPAN8 protein expression: for patients developing or not metastases in the 5 years after diagnosis of their primary melanoma, the number of samples expressing or not Tspan8 protein as well as the percentage of Tspan8+ tumors are presented (n = 100). Fisher’s exact test. e Vital status of the patients according to Tspan8 protein expression: the number of samples expressing or not Tspan8 protein as well as the percentage of Tspan8+ tumors are presented (n = 100). Fisher’s exact test. f 5-years overall survival (n = 100). Log-rank test. g The repartition of tumors according to BRAF status and Tspan8 protein expression is presented. Fisher’s exact test