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Fig. 1 | Molecular Cancer

Fig. 1

From: Peritoneal metastasis of colorectal cancer (pmCRC): identification of predictive molecular signatures by a novel preclinical platform of matching pmCRC PDX/PD3D models

Fig. 1

Matched pmCRC models retain histopathological tumor features and are suitable to determine therapeutic response. A Schematic representation of the project to generate a preclinical platform of matched pmCRC PDX/PD3D models for evaluating treatment response and predictive biomarker signatures. B PDX tumors retain histopathological features of the original human metastastic tissue, determined by H&E staining and KRT19 immunohistochemistry. C Human tumor stroma in pmCRC PDX models is replaced by mouse stroma during passaging, determined by immunofluorescence. D Treatment response of pmCRC PDX models (n = 14). Treatment of PDX models was started at palpable tumors (0.1 cm3) and the ratio of the mean TV of the treated group (T) and the solvent treated control group (C) was expressed as the T/C-value in percent. E Treatment response of pmCRC PD3D cell cultures (n = 9) as viability at highest plasma concentration (Cmax) of each tested compound. Whiskers and outliers are plotted according to Tukey. F Bubble plot representation of categorized treatment response of 9 matched pmCRC PDX and PD3D models (T/C and viability at Cmax, respectively) for treatment with SoC and targeted drugs. The shading of the fields indicates the degree of concordance in response, the color of each circle represents individual drugs and the size of each circle corresponds with the number of models in the same category. T/C-values for PDX models were categorized as strong response (0–10%), moderate response (11–25%), minor response (26–50%) and resistant (> 50%). Similarly, viability of PD3D cell cultures at Cmax was categorized as strong response (0–30%), moderate response (31–60%), minor response (61–80%) and resistant (> 80%)

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