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Fig. 1 | Molecular Cancer

Fig. 1

From: Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer

Fig. 1

Mechanism of the CRISPR/Cas9 gene editing system. The single guide RNA (sgRNA) directs the Cas9 nuclease to a complementary sequence in the genome where Cas9 will induce a double strand break (DSB). The target genomic locus must be followed by a 5′-NGG-3′motif (protospacer adjacent motif, PAM) for Cas9 to function. DSB are repaired by non-homologous end joining (NHEJ), or by homology directed repair (HDR) in the presence of a DNA repair template, which can be exploited to introduce precise genetic modifications or exogenous sequences

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Molecular Cancer

ISSN: 1476-4598