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Fig. 2 | Molecular Cancer

Fig. 2

From: Profiling of DNA damage and repair pathways in small cell lung cancer reveals a suppressive role in the immune landscape

Fig. 2

Targeting DNA pairing and strand exchange process activated the immune contents of SCLC. A, RAD51 inhibition primed SCLC cells to immune checkpoint activation. H446 cells were pretreated with 10 μM RAD51 inhibitor RI-1 for 6 h, then followed by 4Gy IR treatment, 12 h later cells were harvested and RNAs were extracted for qPCR array. Eighty-three genes related to immune checkpoint were amplified by qPCR. B. RAD51 inhibition promoted the migration PBMCs derived from SCLC patients. Migration assay was performed using Transwell chambers. PBMC from SCLC patients were seeded into the upper wells, while the lower chambers contained either RPMI medium or conditioned medium from H446 cells treated with RI-1 or in combination with IR. The results of these experiments are expressed as fold-change vs. control± SEM after 6 h incubation (n = 4). Statistical analysis was performed by ANOVA (*P < 0.05). C. A schematic model of how targeting RAD51 primes the activation of immune contents in the tumor microenvironment. RAD51 mediated DNA pairing and strand exchange interconnects between DSB repair and immune responses

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