Table 1 Summary of the main KRAS detection methods

Direct sequencing

All mutations in the interested region

10–30%

[41]

TheraScreen KRAS kit

7 KRAS mutations in codons 12 and 13

Approximately 1%

[42, 43]

StripAssay

10 KRAS mutations in codons 12 and 13

1%

[44, 45]

SNaPshot

12 KRAS mutations in codons 12 and 13

10%

[44, 45]

Cobas

19 KRAS mutations in codons 12, 13 and 61

Approximately 1%

[46]

Next generation sequencing

All clinical relevant KRAS mutations

1–6%

[47, 48]

Droplet digital PCR

7 KRAS mutations in codons 12 and 13

0.01–0.05%

[48, 49]

BEAMing

16 KRAS mutations in codons 12, 13, 59, 61, 117 and 146

0.01%

[48, 50]