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Fig. 2 | Molecular Cancer

Fig. 2

From: CircCD44 plays oncogenic roles in triple-negative breast cancer by modulating the miR-502–5p/KRAS and IGF2BP2/Myc axes

Fig. 2

CircCD44 promotes the proliferation and chemoresistance of TNBCs. A Establishment of circCD44-stable knockdown and overexpression cell lines using two specific shRNAs or circCD44 plasmid. The relative RNA level was detected in each cell line; ***, P < 0.001. B CCK-8 assay of different cell lines with the indicated modifications; ***, P < 0.001. C Representative images of colony formation assays in different cell lines with the indicated modifications. Statistical analysis is shown in (E); ***, P < 0.001. D Representative image of the EdU assay in different cell lines with the indicated modifications. Statistical analysis is shown in (F). Scale bar: 50 μm; ***, P < 0.001. G The indicated cells were treated with Cyc, and the rate of apoptosis was detected; ***, P < 0.001. H. Immunoblot of the caspase cascade in different cell lines with the indicated modifications. C-C3, cleaved Caspase 3; C-C9, cleaved Caspase 9; C3, Caspase 3; C9, Caspase 9. Data are representative of at least 2–3 experiments with similar results

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