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Fig. 4 | Molecular Cancer

Fig. 4

From: CircCD44 plays oncogenic roles in triple-negative breast cancer by modulating the miR-502–5p/KRAS and IGF2BP2/Myc axes

Fig. 4

CircCD44 acts as an endogenous competing RNA by sponging miRNA miR-502–5p. A Schematic diagram of circCD44, miR-502–5p and the mutant allele. We identified miR-502–5p as a potential target miRNA by searching CircNET, RNAhybrid and miRNAda tools. The interaction site between circCD44 and miR-502–5p was obtained with an online tool (https://circinteractome.nia.nih.gov/). The circCD44 mut allele was established by replacing A with U, U with A, C with G and G with C. B Relative RNA level of miR-502–5p in TNBC cell lines with the indicated modifications using miRNA detection primers; ***, P < 0.001. C FISH of circCD44 and miR-502–5p in the MDA-MB-231 cell line, scale bar: 20 μm. D BT-549 cells were transfected with circCD44 and the mutant allele. RNA pulldown was applied using a junction-specific probe, the complex was subjected to qRT–PCR assay, and then miR-502–5p was detected and normalized; ***, P < 0.001. E The relative RNA level of miR-502–5p was determined in the whole cohort of our in-house TNBC database; ***, P < 0.001. F The correlation between miR-502–5p and circCD44 was evaluated, and regression analysis was applied (r = 0.72, P < 0.001). Data are representative of at least 2–3 experiments with similar results

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