Fig. 4

METTL3/IGF2BP3-downregulated antitumor immunity in breast cancer cells. a The cytotoxicity was measured by lactate dehydrogenase (LDH) release assay after incubation for 48 h. b-c The IFN-γ and IL-2 protein levels in co-culture medium were measured by ELISA after 48 h co-incubation. d-f The PD-1, TIM3 and NR4A1 mRNA expression levels were tested by qRT-PCR. g Images at the end points of subcutaneous xenograft tumors formed by MDA-MB-231 cells in B-NDG mice (n = 5 for each group; scale bar, 1 cm). h Tumors weight were measured in the xenograft mice. Results were presented as mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; n.s., no significance