Skip to main content
Fig. 3 | Molecular Cancer

Fig. 3

From: Cancer cell-derived exosomal circUSP7 induces CD8+ T cell dysfunction and anti-PD1 resistance by regulating the miR-934/SHP2 axis in NSCLC

Fig. 3

Characterization of exosomes and inhibition of CD8+ T cell function by circUSP7 expression. a High-resolution TEM image of NSCLC cell-derived exosomes (scale bar = 100 nm). b The expression of exosomal biomarkers in NSCLC cell-derived exosomes was detected by western blot. c CircUSP7 expression in NSCLC cell-derived exosomes was measured by qRT-PCR. d CircUSP7 expression in plasma exosomes derived from A549 cells was modified by transfection with shRNA to knock down its expression, and circUSP7 expression in plasma exosomes derived from NCI-H460 cells was modified by transfection with cDNA to increase its expression. e The secretion of IFN-γ, TNF-α, Perforin, and Granzyme-B by CD8+ T cells cocultured with exosomes derived from A549 cells transfected with shRNA or exosomes derived from NCI-H460 cells transfected with cDNA was analysed by ELISA. The data are presented as the mean ± SD. **P < 0.01. NS: not significant

Back to article page

Molecular Cancer

ISSN: 1476-4598