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Fig. 4 | Molecular Cancer

Fig. 4

From: Cancer cell-derived exosomal circUSP7 induces CD8+ T cell dysfunction and anti-PD1 resistance by regulating the miR-934/SHP2 axis in NSCLC

Fig. 4

CircUSP7 inhibits the biological function of miR-934 in CD8+ T cells. a RIP was performed for circRNA in CD8+ T cells using a circUSP7 probe and a NC probe. b RIP experiments were carried out in CD8+ T cell extracts using an anti-AGO2 antibody. c The level of circUSP7 in the streptavidin-captured fractions from CD8+ T cell lysates after transfection with biotinylated miR-934 or the NC. CircANRIL was used as the negative control. d Putative binding sites of miR-934 in the circUSP7 sequence were predicted by StarBase v3.0. e The luciferase activity of pGL3-circUSP7 in CD8+ T cells after cotransfection with miR-934. f CircUSP7 expression in CD8+ T cells cocultured with exosomes derived from A549 cells and NCI-H460 cells. g The relative level of miR-934 in CD8+ T cells transfected with NC, shcircUSP7, mock, or circUSP7 was measured by qRT-PCR. The data are presented as the mean ± SD; **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598