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Fig. 5 | Molecular Cancer

Fig. 5

From: Cancer cell-derived exosomal circUSP7 induces CD8+ T cell dysfunction and anti-PD1 resistance by regulating the miR-934/SHP2 axis in NSCLC

Fig. 5

CircUSP7 regulates the miR-934/SHP2 pathway in CD8+ T cells. a The putative binding site of miR-934 in the SHP2 sequence was predicted by StarBase v3.0. b Luciferase activity of pGL3-SHP2 in CD8+ T cells cotransfected with miR-934. c and d The mRNA and protein levels of SHP2 were measured by qRT-PCR and western blotting, respectively, in CD8+ T cells transfected with miR-934 or miR-934 combined with circUSP7. e SHP2 in representative NSCLC patients was analysed by IHC staining. f A positive correlation between circUSP7 and SHP2 mRNA expression was observed in the CD8+ T cells from NSCLC patients (R2 = 0.6580; P < 0.0001). g A negative correlation between circUSP7 and miR-934 expression was observed in the CD8+ T cells from NSCLC patients (R2 = 0.2470; P = 0.0052). h A negative correlation between miR-934 and SHP2 mRNA expression was observed in the CD8+ T cells from NSCLC patients (R2 = 0.2330; P = 0.0069). The data are presented as the mean ± SD; *P < 0.05, **P < 0.01, NS: not significant

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Molecular Cancer

ISSN: 1476-4598