Fig. 1

Identification of has_circ_0020303 in ccRCC tissues. A Volcano plot of GSE137836 and GSE100186. Compared to the primary tumors (in GSE137836) and adjacent normal tissue (in GSE100186), red dots represent significantly up-regulated circRNAs, while green dots represent significantly down-regulated circRNAs. Grey dots represent circRNAs that are not significant. B Venn plot of the two datasets. Common circRNAs with p < 0.05, |log2 (fold change)| > 1 are chosen. C identification of sixteen circRNAs from the overlaps of top 100 DEGs in two circRNA microarrays. D Heat map shows dysregulated circRNAs between ccRCC tissues (T) and adjacent noncancer tissues (N). The numerical data represented the serial number of circRNAs in circBase. E Heat map shows dysregulated circRNAs between metastatic ccRCC tissues and primary ccRCC tissues. F a ceRNA network was established based on circRNA-miRNA and miRNA-mRNA intersections. G Expression of circRNA hsa_circ_0020303 in pan-cancer analysis or in ccRCC tissues and adjacent noncancer tissues detected by qRT-PCR (N = 175). H Correlation between circRNA hsa_circ_0020303 and clinical characteristics. I Overall survival and progression-free survival analysis of ccRCC patients with high hsa_circ_0020303 expression and low levels of hsa_circ_0020303