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Fig. 2 | Molecular Cancer

Fig. 2

From: circCHST15 is a novel prognostic biomarker that promotes clear cell renal cell carcinoma cell proliferation and metastasis through the miR-125a-5p/EIF4EBP1 axis

Fig. 2

The validation and characteristics of circCHST15 in RCC cells. A Relative expression of circCHST15 in the human renal proximal tubular epithelial cell line HK2immortalized uroepithelium cell line HK2, and RCC cell lines 786-O, 769P, ACHN, CAKI-1, A498 and OSRC2. B qRT-PCR analysis of circCHST15 in the reverse transcription products using random primers or oligo dT primers. C The existence of circ CHST15 was detected in 786-O cell lines by qRT-PCR with convergent or divergent primers and validated by Gel electrophoresis. D The expression of circCHST15 was validated by Sanger sequencing. Yellow arrow represents the back-splicing site of circCHST15. CircCHST15 derived from back-splicing of exons 2,3, 4,5,6,7,8 and 9 of CHST15 gene. E qRT-PCR analysis of circ CHST15 and CHST15 mRNA after treatment with Actinomycin D at the indicated time points in 786-O cells. F qRT-PCR analysis of circ CHST15 and CHST15 mRNA after treatment with or without RNase R in 786-O and CAKI-1 cells. G qRT-PCR analysis of circCHST15 using nuclear and cytoplasmic fractions of 786-O and CAKI-1 cells. H FISH confirmed that circCHST15 was predominantly located in cytoplasm. Nuclei were stained with DAPI. U6, 18S and circCHST15 were labeled with Cy3. K-L Influence of hsa_circ_0020303 on cell proliferation of ccRCC cells detected by 5-ethynyl-2′-deoxyuridine (EdU) staining assay

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