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Fig. 5 | Molecular Cancer

Fig. 5

From: circCHST15 is a novel prognostic biomarker that promotes clear cell renal cell carcinoma cell proliferation and metastasis through the miR-125a-5p/EIF4EBP1 axis

Fig. 5

Up-regulated/down-regulated miR-125a-5p suppresses/promotes cell proliferation, migration and invasion through targeting EIF4EBP1 in vitro. A Relative expression of miRNA-125a-5p in HK2, 786-O, CAKI1 and A498 cell lines. B-E 786-O and CAKI-1 cells transfected with mimics NC or miR-125a-5p mimics. F-I, A498 and HK2 cells transfected with Inhibitor NC or miR-125a-5p Inhibitor. B, F Cell proliferation ability of RCC cells and HK2 cells was evaluated by CCK8 assay. C, G Cell migration capability of RCC cells and HK2 cells was evaluated by wound healing assays. D, H Cell proliferation ability of RCC cells and HK2 cells was evaluated by colony formation assay. E, I The influence on cell migration and invasion abilities of RCC cells and HK2 cells was assessed by transwell migration and matrigel invasion assays. J Luciferase reporter assay in HEK293T cells co-transfected with miRNA mimics, psiCHECK-2-wild type EIF4EBP1 (EIF4EBP1-wt) or psiCHECk-2-mutant type EIF4EBP1 (EIF4EBP1-mut) plasmids. K Schematic of EIF4EBP1 wild-type (wt) and mutant (mut) luciferase reporter vectors. L Western blot analysis indicated that miR-125a-5p could down-regulate EIF4EBP1 expression in RCC cells; histogram for protein expressions among different groups. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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