Skip to main content
Fig. 2 | Molecular Cancer

Fig. 2

From: Remodeling of the tumor microenvironment via disrupting Blimp1+ effector Treg activity augments response to anti-PD-1 blockade

Fig. 2

Delayed tumor growth and enhanced anti-tumor effector responses in mice with Foxp3-specific deletion of Blimp1. Blimp1 WT (Foxp3YFP-Cre), Het (Prdm1fl/+Foxp3YFP-Cre) and KO (Prdm1fl/flFoxp3YFP-Cre) mice were inoculated with MC38 (n = 6 per group) (a), B16-F10 (WT: n = 4; KO: n = 6) (b) or B16-OVA at day 0 and immunized with NP-OVA at days 0, 7 (as Fig. 1a) (WT: n = 6; KO: n = 14) (c) or B16-OVA at day 0 and vaccinated with GVAX at days 1, 3 and 7 (WT: n = 4; Het: n = 4; KO: n = 6) (d), and tumor growth was monitored. e) Analysis of IFNγ, TNFα and GzmB expression in TIL CD4+Foxp3 Teff, CD8+ T-cells and NK cells from mice as in c. f) Analysis and quantitation of TIL CD11c+MHCII+ DC (WT: n = 3; KO: n = 4). g) Expression of MHCII and CD206 on TIL F4/80+ macrophages, and ratio of MHCII+M1:CD206+M2 (WT: n = 3; KO: n = 4). Data represent one of two (a-b,d,f-g) or at least three (e) or are pooled from two (c) independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 (a-d, two-way ANOVA with sidak’s comparisons test (a-c) or with tukey’s test (d), black: KO compared to WT, red: Het compared to WT; f,g, unpaired two-tailed Student’s t-test). Bars, mean ± SEM

Back to article page