Fig. 3

TIL Blimp1-deficient Treg cells convert into effector T-cells and display impaired suppressive function. a-c) Analysis of TIL Treg cells (n = 7 per group) from mice as in Fig. 2c. Quantitation of frequency and Foxp3 MFI of Treg cells (a); IFNγ, TNFα and GzmB expression in Treg cells (b) or frequency of TIL Treg cells expressing these molecules (c). d) B16-OVA/GVAX model was established as in Fig. 2d. IFNγ, TNFα and GzmB expression in TIL Treg cells (n = 4 per group). e) In vitro suppression of CTV-labelled CD8⁺ T-cell proliferation performed in duplicates per experimental group. Percent suppression is shown. WT: Foxp3^YFP-Cre, KO: Prdm1^fl/flFoxp3^YFP-Cre. SP: spleen; TU: tumor. Data represent one of at least three (a-c) or two (d-e) independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 (a,c,d,e, unpaired two-tailed Student’s t-test). Bars, mean ± SEM