Fig. 7

Deletion of Eomes in Blimp1-deficient Treg cells promotes tumor growth. a) Comparison and quantitation of Eomes levels in TIL Treg cells from B16-OVA/NP-OVA mice (n = 8 per group), as in Fig. 2c. b) B16-OVA model was established in each mouse strain (WT: n = 7; KO: n = 6; DKO: n = 5), as in Fig. 2c. Tumor sizes are shown. c) Comparison ad quantitation of Eomes MFI in TIL Treg cells from b. The vertical dotted line represents the threshold for the gating of Eomes⁺ cells. d) Frequency of TIL Treg and T_FR cells (PD-1⁺Bcl6⁺Foxp3⁺CD4⁺CD3⁺) and Foxp3 MFI of Treg cells as well as TIL Treg cells expressing IFNγ and GzmB or CD8⁺ T-cells expressing GzmB. e) Frequency of TIL T_FH (PD-1⁺Bcl6⁺Foxp3⁻CD4⁺CD3⁺) and GC B-cells (GL-7⁺Fas⁺CD19⁺). f) Serum total IgG, IgE and anti-OVA IgG, IgE titers. The value 0 is used to indicate the undetectable anti-OVA IgE titers. In c-f, WT: n = 5-6; KO: n = 4-5; DKO: n = 5-8. Iso: Isotype control. WT: Foxp3^YFP-Cre, KO: Prdm1^fl/flFoxp3^YFP-Cre, DKO: Eomes^fl/flPrdm1^fl/flFoxp3^YFP-Cre. ∆MFI: MFI subtracted from the MFI of isotype controls. Data are pooled from two (a) or represent one of two independent experiments (b-f). ns, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.001 (a,c,d-f, unpaired two-tailed Student’s t-test; b, two-way ANOVA with Tukey’s comparisons test, black: compared to WT, red: compared to KO). Bars, mean ± SEM