Fig. 2

CircDOCK1 promotes the malignant transformation of OS cells. A circDOCK1 RNA expression was measured by qRT–PCR post transfection of the overexpression plasmid, siRNA1 and siRNA2 (normalized to si-NC or vector). B CCK-8 assay assessing MG63 and U2OS cell proliferation post transfection with circDOCK1 siRNAs or the overexpression plasmid. C EdU incorporation assays assessing MG63 cell proliferation (scale bar = 100 μm). D Colony formation assays (normalized to si-NC or vector). E Flow cytometry detecting the influence of circDOCK1 on cell apoptosis. F-G Transwell assays detecting the influence of circDOCK1 on cell migration (F) and invasion (G) (normalized to si-NC or vector, scale bar = 100 μm). The data are presented as the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001, *vs. si-NC, ^#vs. vector